The surplus of embryos is not clinically used or is unsuitable for implantation into the patient, and therefore may be donated by the donor with consent. Human embryonic stem (hES) cells must be monitored and cared for in order to maintain healthy, undifferentiated cultures. Therefore, scientists have devised special culturing techniques to maintain or take advantage of these properties. At minimum, the cultures must be fed every day by performing a complete medium change to replenish lost nutrients and to keep the cultures free of unwanted differentiation factors.

Embryonic stem cells, or ES cells, have a number of unique properties that distinguish them from regular adult cells. When cultured properly, ES cells can divide indefinitely to make more of themselves. In vitro fertilization generates multiple embryos. Although a small amount of differentiation is normal and expected in … Human embryonic stem (hES) cells must be monitored and cared for in order to maintain healthy, undifferentiated cultures. The inner cell mass (cells of interest), from the blastocyst stage of the embryo, is separated fro… Protocol: Human Embryonic Stem Cell Culture Basement membranes are continuous sheets of specialized extracellular matrix that are found at the dermal-epidermal junction, at the base of all lumen-lining epithelia throughout the digestive, respiratory, reproductive, and urinary tracts, and that underlie parenchyma of endo- crine and exocrine glands. Human embryonic stem cells can be derived from these donated embryos or additionally they can also be extracted from cloned embryos using a cell from a patient and a donated egg.

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